bslA [2017-07-13 10:10:00]

bacterial hydrophobin, forms water-repellent surface layer of the biofilm, required for sliding, inhibitor of KinA autophosphorylation, and subsequently of entry into sporulation

locus: BSU31080

pI: 9.99

mw: 19.11 kDa

protein length: 181 aa

gene length: 543 bp

function: biofilm formation, control of entry into sporulation via the phosphorelay

product: biofilm surface layer, inhibitor of KinA autophosphorylation

essential: no

synonyms: yuaB, sivB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.2|Proteins controlling the activity of the kinases]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.3|Repellent surface layer]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.2|Proteins controlling the activity of the kinases]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 3,187,503 → 3,188,048

Phenotypes of a mutant

• reduced colony complexity [Pubmed|21742882]
• loss of surface repellency of the biofilm [Pubmed|22571672]
• diminished [SW|sliding] of ''B. subtilis'' natto [Pubmed|26152584]

The protein

Catalyzed reaction/ biological activity

• inhibits [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|KinA] autophophorylation [Pubmed|23335417]
• contributes to the surface stiffness and increases the surface roughness of NCIB 3610 bioflms [Pubmed|26873313]

Paralogous protein(s)

• [protein|E5E2994D96FCECEE75E15136A08308C7B6C1C183|SivA]

Structure

• [PDB|4BHU] [Pubmed|23904481]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]
• biofilm matrix in an extracellular polysaccharide-dependent manner [Pubmed|22571672]
• forms a layer on the biofilm surface [Pubmed|22571672]

Expression and Regulation

Operons

• genes: [gene|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|bslA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|18978066], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|LutR]: activation, [Pubmed|24196425], in [regulon|E6F3BAC5875A16D8D75B2AD21447C14DD17B3DA6|LutR regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|18978066], this might be indirect [Pubmed|21742882], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]

Biological materials

Mutant

• MGNA-A218 (yuaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/218 NBRP B. subtilis, Japan]
• ''B. subtilis'' W939 ''bslA''::Cm (ATCC6051 based) [Pubmed|17850253]
• ''B. subtilis'' NRS2097 ''bslA''::Cm (NCIB3610 based) [Pubmed|18978066]
• ''B. subtilis'' ''bslA''::Cm (168 based) [Pubmed|21097620]

Expression vector

• Physpank-bslA based on vector pDR111: pDRyuaB2 [Pubmed|21097620]

lacZ fusion

• pNW500 P''bslA-lacZ'' fusion in pDG1663 [Pubmed|18978066]

GFP fusion

• P''bslA-gfp'' fusion in pSG1151 vector: pSGyuaB [Pubmed|21097620]

References

Reviews

• 22607588,23979433,24988880,25907113,27458867

Original publications

• 27298433,22571672,18978066,18957862,17850253,21097620,21742882,22383849,23335417,23904481,22571672,25870300,26152584,26378478,26873313